Absorbance range for spectrophotometry

Закрыть ... [X]


Spectrophotometry, branch of that deals with measurement of the transmitted or reflected by a body as a function of the wavelength. Ordinarily the intensity of the energy transmitted is compared to that transmitted by some other system that serves as a standard. Different types of modern spectrophotometers cover wide ranges of the electromagnetic spectrum: , ultraviolet, visible, infrared, or microwave.

Two laws express the relationship between the absorption of radiant energy and the absorbing medium. According to Bouguer’s (or Lambert’s) law, each layer of equal thickness of the medium absorbs an equal fraction of the energy it. According to , the absorptive capacity of a dissolved substance is directly proportional to its concentration in a solution.

Ultraviolet spectrophotometry is particularly useful in detecting colourless substances in solution and measuring their concentration. Infrared spectrophotometry is most commonly used in studying the molecular structures of complex organic .

Learn More in these related Britannica articles:

  • Synthesis of protein.

    protein: Spectrophotometric behaviour

    Spectrophotometry of protein solutions (the measurement of the degree of absorbance of light by a protein within a specified wavelength) is useful within the range of visible light only with proteins that contain coloured prosthetic groups (the nonprotein components). Examples of such proteins include the…

  • pH paper

    chemical analysis: Absorptiometry

    …assay are absorptiometry or absorption spectrophotometry.…

  • photolysis

    …means for their identification by spectrophotometry. The method is a valuable tool for the identification of transient chemical intermediates and hence for the study of mechanisms of fast chemical reactions.…

  • Beer's law

    Beer’s law, in spectroscopy, a relation concerning the absorption of radiant energy by an absorbing medium. Formulated by German mathematician and chemist August Beer in 1852, it states that the absorptive capacity of a dissolved substance is directly proportional to its concentration in…

More About Spectrophotometry

3 references found in Britannica articles

Assorted References

  • chemical analysis
  • photolytic processes
  • proteins



Stretching and bending vibrations in organic compounds such as 5-hexene-2-one represent different energy levels within a molecule that can be detected by using infrared spectrophotometry. key people related topics


Related News

The print shop photo pro
Men in the shower photos
Baby girl photo album
Photo story 6 free download
Www eenadu net photos
South actress photo download